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Author Topic: The CTRL-V Game!  (Read 59972 times)

BlackDragonSlayer

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Re: The CTRL-V Game!
« Reply #705 on: March 23, 2019, 12:30:17 AM »

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And the moral of the story: Quit while you're a head.

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Libera

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Re: The CTRL-V Game!
« Reply #706 on: April 21, 2021, 02:53:58 PM »

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Re: The CTRL-V Game!
« Reply #707 on: April 21, 2021, 03:07:32 PM »

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Maelstrom

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Re: The CTRL-V Game!
« Reply #708 on: April 21, 2021, 05:03:48 PM »

3.6 – CTF Correction
Point spread function – PSF
-If you had a single point and imaged it, due to inherent issues, point is blurred, maybe surrounded by a halo
This function is true for each point before CTF isues
You can use stuff like gold beads which are single points and then are surrounded by halos and peaks
PSF is a Fourier transform of the CTF (and vice versa)
Remember, the Fourier transform of a gaussian function is another gaussian function
A broad G function because a narrow one, vice versa
A wave becomes a curve with bumps afterwards
PSF is identical for all points of high density in the image
--Called convolution
Image = Object (convolved with) PSF
Image = inverse fourier transform(Fourier transform of the object * CTF)
Here we go
Object = Inv F tfm ( F tfm (image)/CTF)
So, if the CTF is known, then math can be used to pull it back out
basically, take the FT of the image, divide by CTF, and then inverse FT and you will get the object
well, a closer version
Called CTF correction
Can’t multiply by zero when CTF crosses axis
So, can just exclude those
Or, can “phase flip” elements that have a negative phase in the CTF
To do all this, you need to fully understand the CTF

Libera

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Re: The CTRL-V Game!
« Reply #709 on: April 21, 2021, 05:08:42 PM »

Remember, the Fourier transform of a gaussian function is another gaussian function

Wasn't expecting to read such important facts here.

mastersuperfan

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Re: The CTRL-V Game!
« Reply #710 on: April 21, 2021, 05:30:07 PM »

phosphatidylinositol
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there's also a huge difference in quality between 2000 songs and 2010 songs
The difference between 2000 songs and 2010 songs is 10 songs.

BlackDragonSlayer

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Re: The CTRL-V Game!
« Reply #711 on: April 23, 2021, 02:18:50 PM »

Research Article Summary
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Re: The CTRL-V Game!
« Reply #712 on: April 23, 2021, 03:01:16 PM »

Keratosis pilaris

(Turns out I have it. Good thing it's not dangerous)
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Re: The CTRL-V Game!
« Reply #713 on: April 23, 2021, 07:50:15 PM »

Ph'nglui mglw'nafh Cthulhu R'lyeh wgah'nagl fhtagn
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Re: The CTRL-V Game!
« Reply #714 on: April 23, 2021, 08:00:41 PM »

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play len'en and kid icarus uprising

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Re: The CTRL-V Game!
« Reply #715 on: April 24, 2021, 07:40:06 PM »

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tfw you get schooled in English grammar by a guy whose first language is not English

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Re: The CTRL-V Game!
« Reply #717 on: May 04, 2021, 01:23:10 AM »

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BlackDragonSlayer

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Re: The CTRL-V Game!
« Reply #718 on: May 06, 2021, 05:01:01 PM »

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And the moral of the story: Quit while you're a head.

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